3'-dInosine 40 nmol scale, 1 modification

Inosine is a nucleoside that occurs naturally in the wobble position of the anticodon of some transfer RNAs and is known to form base pairs with A, C and U during the translation process. Several experiments have suggested that deoxyinosine might be an “inert” base, its presence in an oligonucleotide sequence seems neither to disturb DNA duplex formation nor to destabilize the duplex. Independent of sequence effects, the order of base-pairing stabilities is I-C >> I-A > I-T = I-G. Deoxyinosine containing primers have been used successfully to amplify cDNA or genomic fragments for the generation of DNA probes from a peptide sequence.