08-24-00001-5, HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX), 5 ml, 1250 rxn

Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. In this mix EvaGreen® double-stranded DNA binding dye is used instead of the more widely used SYBR Green I that has similar fluorescence spectra. Compared to SYBR Green I dye EvaGreen® dye shows a higher fluorescence level, high sensitivity for detecting low template concentrations, and high stability at room temperature.

HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimized ready-to-use solution for dye-based real-time quantitative PCR assays on cyclers that require passive reference dye (including high ROX or low ROX reference signal requiring platforms).

Applications

Detection and quantification of DNA and cDNA targets

Profiling gene expression

Microbial detection

Viral load determination

Properties

Concentration: 5x

Hot-start: yes, initial activation in 12-15 min

Detection type: dye-based, includes EvaGreen® intercalating dye

Reference dye: based on ROX

Compatible real-time instruments: Cyclers that require ROX reference dye. Check Your cycler!

Mix Components

HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start

5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2

dNTPs: dATP, dCTP, dGTP and dTTP

EvaGreen® dye

Internal reference based on ROX dye

EvaGreen Dye

EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is an extremely stable dye, and has been shown to be non-mutagenic and non-cytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!