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08-47-00020, HOT FIREPol® SolisGreen qPCR Mix 2.0, 20 ml, 5000rxn
10.301,83 RON
Highly sensitive dye-based qPCR mix with improved detection of low-copy targets
- Great PCR efficiency – reliable and easy-to-analyze results
- High sensitivity – great when working with low concentrated samples
- Excellent fluorescence levels
- Convenient reaction set-up at room temperature
- Environmentally friendly – worldwide ice-free shipping
SKU
SBD_08-47-00020
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is a 5x-concentrated solution specially designed for dye-based real-time quantitative PCR (qPCR) assays. SolisGreen® dsDNA binding dye is incorporated into the product to evaluate the DNA amplification process during PCR. The fluorescence spectra of this dye are similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. When compared to SYBR® Green I, SolisGreen® dye shows:
- higher fluorescence level
- better sensitivity for detecting low template concentrations
- great stability for room temperature storage
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is an optimized ready-to-use solution that contains all the necessary components, except sample (DNA template) and primers, to perform reactions with accurate and highly sensitive results. Additionally, the product includes a passive reference based on ROX dye making it compatible with both ROX-dependent and ROX-independent qPCR cyclers.
The product contains HOT FIREPol® DNA Polymerase to prevent the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Applications
- Gene expression analysis and absolute quantification
- Pathogen detection and quantification
- Low-copy gene detection
- Cell-free DNA analysis
For RNA applications try out an already optimized easy-to-use SOLIScript® 1-step SolisGreen® Kit 2.0 or combine HOT FIREPol® SolisGreen® qPCR Mix 2.0 with thermostable reverse transcriptases, such as FIREScript® and SOLIScript®!
Properties
Sample type: DNA
Concentration: 5x
Hot-start: Yes, initial activation in 10 min
Detection type: Dye-based, includes SolisGreen® intercalating dye
Reference dye: Based on ROX
Compatible real-time instruments: The mix is compatible with both ROX-dependent and ROX-independent qPCR cyclers. HOT FIREPol® SolisGreen qPCR Mix 2.0 is not compatible with platforms that require high ROX, such as Applied BioSystems® 7900HT, StepOne™ or StepOnePlus™ systems.
Mix Components
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start.
5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
dNTPs: dATP, dCTP, dGTP and dTTP
SolisGreen® dye
Internal reference based on ROX dye
| Price | 8.657,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is a 5x-concentrated solution specially designed for dye-based real-time quantitative PCR (qPCR) assays. SolisGreen® dsDNA binding dye is incorporated into the product to evaluate the DNA amplification process during PCR. The fluorescence spectra of this dye are similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. When compared to SYBR® Green I, SolisGreen® dye shows:
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is an optimized ready-to-use solution that contains all the necessary components, except sample (DNA template) and primers, to perform reactions with accurate and highly sensitive results. Additionally, the product includes a passive reference based on ROX dye making it compatible with both ROX-dependent and ROX-independent qPCR cyclers. The product contains HOT FIREPol® DNA Polymerase to prevent the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Applications
For RNA applications try out an already optimized easy-to-use SOLIScript® 1-step SolisGreen® Kit 2.0 or combine HOT FIREPol® SolisGreen® qPCR Mix 2.0 with thermostable reverse transcriptases, such as FIREScript® and SOLIScript®! Properties Sample type: DNA Concentration: 5x Hot-start: Yes, initial activation in 10 min Detection type: Dye-based, includes SolisGreen® intercalating dye Reference dye: Based on ROX Compatible real-time instruments: The mix is compatible with both ROX-dependent and ROX-independent qPCR cyclers. HOT FIREPol® SolisGreen qPCR Mix 2.0 is not compatible with platforms that require high ROX, such as Applied BioSystems® 7900HT, StepOne™ or StepOnePlus™ systems. Mix Components HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start. 5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP SolisGreen® dye Internal reference based on ROX dye
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