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08-59-00250, SOLIScript® 1-step Multiplex Probe Kit (ROX) (250 rxn)
2.388,93 RON
Simple and highly specific cDNA synthesis and qPCR in a single tube
Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip® RNase inhibitor, and nuclease-free water.
- cDNA synthesis up to 60°C for superior specificity
- analyze 1-4 targets in 1 reaction
- reaction set-up and shipment without dry ice
- robust amplification of GC-rich targets
- contains dUTP to prevent cross-contamination when used in combination with UNG
- RNase inhibitor included in kit
- Includes ROX reference dye
SKU
SBD_08-59-00250
SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.
Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays.
Applications
- Detection and quantification of RNA targets with qPCR
- DNA/LNA hydrolysis probe based assays
- Suitable for singleplex and multiplex reactions
Properties
Sample type: RNA
Product format: Reagent kit
Detection type: Probe-based
Reference dye: ROX
Compatible real-time instruments: The mix is compatible with qPCR cyclers that require an internal reference dye (e.g. ROX) for normalization of fluorescent signal.
Reagents
5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), ROX dye (If ROX dye is used as one of the fluorophpres, internal passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available)
40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip® RNase Inhibitor
Water, nuclease free
| Price | 2.007,50 RON (preturile sunt fara TVA) |
|---|---|
| Description |
SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube. Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays. Applications
Properties Sample type: RNA Product format: Reagent kit Detection type: Probe-based Reference dye: ROX Compatible real-time instruments: The mix is compatible with qPCR cyclers that require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Reagents 5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), ROX dye (If ROX dye is used as one of the fluorophpres, internal passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available) 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip® RNase Inhibitor Water, nuclease free
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