Limba
Romanian
Romanian
English|
08-01-00001-5, HOT FIREPol® Multiplex qPCR Mix, 5 ml, 1250 rxn
3.528,74 RON
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction
Probe-based qPCR master mix that has been optimized for highly sensitive and accurate quantification of up to 4 targets in a single reaction. This master mix was developed for TaqMan® probes but is also suitable for other hydrolysis probe types
- analyze 1-4 targets in 1 reaction
- high specificity and sensitivity
- robust amplification of GC-rich targets
- contains dUTP to prevent cross-contamination when used in combination with UNG
SKU
SBD_08-01-00001-5
HOT FIREPol® Multiplex qPCR Mix is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes, and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, and MgCl2.
HOT FIREPol® Multiplex qPCR Mix is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Applications
- detection and quantification of DNA and cDNA targets
- profiling gene expression
- microbial detection
- viral load determination
Properties
Concentration: 5x
Hot-start: yes, initial activation in 10 min
Detection type: Probe-based
Reference dye: none
Suitable qPCR Cyclers: all cyclers that do not need passive reference dye.
Mix Components
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs.
IMPORTANT: UNG is not included in the HOT FIREPol® Multiplex qPCR Mix
| Price | 2.965,33 RON (preturile sunt fara TVA) |
|---|---|
| Description |
HOT FIREPol® Multiplex qPCR Mix is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes, and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, and MgCl2.
Applications
Properties Concentration: 5x Hot-start: yes, initial activation in 10 min Detection type: Probe-based Reference dye: none Suitable qPCR Cyclers: all cyclers that do not need passive reference dye. Mix Components HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3mM MgCl2 dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs.
|
