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06-35-00200, SOLIScript® RT cDNA synthesis KIT(200 rxn)
3.717,56 RON
Superior specificity with gene-specific primers in flexible kit format
Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature.
- superior specificity with gene-specific primers
- wide reaction temperature 37°C - 60°C
- reaction set-up and shipment without dry ice
- in flexible 6-tube kit format
SKU
SBD_06-35-00200
SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.
SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C).
SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.
Applications
- First strand cDNA synthesis
- RT-PCR
- RT-qPCR
Properties
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme with all components included reagent kit
Enzyme: SOLIScript® Reverse Transcriptase
Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents
SOLIScript® Reverse Transcriptase
RiboGrip® RNase Inhibitor (220 U/μl)
10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT
Oligo (dT) Primer (100 μM)
dNTP MIX (20 mM of each)
Water, nuclease free
| Price | 3.124,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase. SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C). SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity. Applications
Properties Sample type: RNA Final product: cDNA (first strand) Product format: Enzyme with all components included reagent kit Enzyme: SOLIScript® Reverse Transcriptase Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene. Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers. Reagents SOLIScript® Reverse Transcriptase RiboGrip® RNase Inhibitor (220 U/μl) 10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT Oligo (dT) Primer (100 μM) dNTP MIX (20 mM of each) Water, nuclease free
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