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06-37-00100, SOLIScript® RT cDNA synthesis MIX (100 rxn)
1.796,85 RON
Superior specificity with gene-specific primers in convenient mix format
Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature.
- superior specificity with gene-specific primers
- wide reaction temperature 37°C - 60°C
- reaction set-up and shipment without dry ice
- in convenient 3-tube kit format
SKU
SBD_06-37-00100
SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.
SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C).
SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.
Applications
- First strand cDNA synthesis
- RT-PCR
- RT-qPCR
Properties
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme mix with all reagents included
Enzyme: SOLIScript® Reverse Transcriptase
Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents
SOLIScript® Enzyme Mix with SOLIScript® RT and RiboGrip® RNase Inhibitor
10x RT Reaction Premix without primers, RT reaction buffer with DTT and dNTPs
Water, nuclease free
| Price | 1.485,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase. SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C). SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity. Applications
Properties Sample type: RNA Final product: cDNA (first strand) Product format: Enzyme mix with all reagents included Enzyme: SOLIScript® Reverse Transcriptase Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene. Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers. Reagents SOLIScript® Enzyme Mix with SOLIScript® RT and RiboGrip® RNase Inhibitor 10x RT Reaction Premix without primers, RT reaction buffer with DTT and dNTPs Water, nuclease free
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