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08-02-00001-10, HOT FIREPol® Multiplex qPCR Mix (ROX), 10 ml, 2500 rxn
6.706,01 RON
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction
Probe-based qPCR master mix that has been optimized for highly sensitive and accurate quantification of up to 4 targets in a single reaction. This master mix was developed for TaqMan® probes but is also suitable for other hydrolysis probe types
- analyze 1-4 targets in 1 reaction
- high specificity and sensitivity
- robust amplification of GC-rich targets
- contains dUTP to prevent cross-contamination when used in combination with UNG
SKU
SBD_08-02-00001-10
HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, MgCl2 and Purple passive reference dye according to system requirements.
HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Applications
- detection and quantification of DNA and cDNA targets
- profiling gene expression
- microbial detection
- viral load determination
Properties
Concentration: 5x
Hot-start: yes, initial activation in 10 min
Detection type: Probe-based
Reference dye: ROX
Suitable qPCR Cyclers: qPCR platforms from Applied Biosystems and Agilent/Stratagene.
Mix Components
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs.
IMPORTANT: UNG is not included in the HOT FIREPol® Multiplex qPCR Mix
ROX dye: If ROX, Texas Red, or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
| Price | 5.635,30 RON (preturile sunt fara TVA) |
|---|---|
| Description |
HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, MgCl2 and Purple passive reference dye according to system requirements.
Applications
Properties Concentration: 5x Hot-start: yes, initial activation in 10 min Detection type: Probe-based Reference dye: ROX Suitable qPCR Cyclers: qPCR platforms from Applied Biosystems and Agilent/Stratagene. Mix Components HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3mM MgCl2 dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. ROX dye: If ROX, Texas Red, or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available. |
