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08-15-00020, HOT FIREPol® Probe qPCR Mix Plus (no ROX), 20 ml, 5000 rxn

08-01-00001, HOT FIREPol® Multiplex qPCR Mix, 1 ml, 250 rxn

08-15-00020, HOT FIREPol® Probe qPCR Mix Plus (no ROX), 20 ml, 5000 rxn

9.598,90 RON

Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry

Cost-effective real-time qPCR master mix for probe-based qPCR assays.
This master mix has been developed for TaqMan^® probes but is suitable for other hydrolysis probe types as well.

suitable for singleplex and duplex assays
high specificity and sensitivity
reaction set-up and shipment without dry ice

SKU

SBD_08-15-00020-1

Detalii

HOT FIREPol^® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe.

The qPCR Mix contains optimized components and HOT FIREPol^® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol^® Probe qPCR Mix Plus (no ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity.

HOT FIREPol^® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications

Detection and quantification of DNA and cDNA targets
Profiling gene expression
Microbial detection
Viral load determination

Properties

Concentration: 5x

Hot-start: yes, initial activation in 12-15 min

Detection type: Probe-based

Reference dye: none

Compatible real-time instruments: cyclers that do not need ROX reference dye.

Mix Components

HOT FIREPol^® DNA polymerase: chemically modified FIREPol^® DNA Polymerase enabeling hot-start

5x Probe qPCR buffer with 15 mM MgCl₂: 1x PCR solution – 3 mM MgCl₂

dNTPs: dATP, dCTP, dGTP and dTTP

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Price	8.066,30 RON (preturile sunt fara TVA)
Description	HOT FIREPol^® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol^® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol^® Probe qPCR Mix Plus (no ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity. HOT FIREPol^® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Applications Detection and quantification of DNA and cDNA targets Profiling gene expression Microbial detection Viral load determination Properties Concentration: 5x Hot-start: yes, initial activation in 12-15 min Detection type: Probe-based Reference dye: none Compatible real-time instruments: cyclers that do not need ROX reference dye. Mix Components HOT FIREPol^® DNA polymerase: chemically modified FIREPol^® DNA Polymerase enabeling hot-start 5x Probe qPCR buffer with 15 mM MgCl₂: 1x PCR solution – 3 mM MgCl₂ dNTPs: dATP, dCTP, dGTP and dTTP

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