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04-36-02020, HOT FIREPol® MultiPlex Mix Ready To Load with 10 mM MgCl2, 20 ml, 5000 rxn
9.458,83 RON
Hot-start master mix for high throughput applications with ready-to-load feature
- analyze multiple targets per reaction
- increased sensitivity and yield
- prevents primer dimer formation
- reaction set-up and shipment without dry ice
SKU
SBD_04-36-02020
HOT FIREPol® MultiPlex Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers, and water) and an additional compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis.
Generated PCR products are compatible with T/A cloning procedures.
Recommendations
We recommend using HOT FIREPol® MultiPlex Mix Ready To Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining.
HOT FIREPol® MultiPlex Mix Ready To Load is not recommended for use in applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.
Properties
Concentration: 5x
Hot-start: yes, initial activation in 12-15 min
Ready to load: yes
Fidelity: 1 x Taq
Cloning Type: T/A cloning
Multiplexing: tested with up to 18 targets (depending on amplicon length)
Amplicon Size: up to 5 kb
Mix Components
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x Multiplex Buffer with 10 mM MgCl2: 1x PCR solution – 2 mM MgCl2
dNTPs: dATP, dCTP, dGTP and dTTP
Bovine serum albumin (BSA) to enhance PCR reaction
Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment
Yellow dye: migration equivalent to 35-45 bp DNA fragment
Compound that increases sample density for direct loading
| Price | 7.948,60 RON (preturile sunt fara TVA) |
|---|---|
| Description |
HOT FIREPol® MultiPlex Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers, and water) and an additional compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis. Generated PCR products are compatible with T/A cloning procedures. Recommendations We recommend using HOT FIREPol® MultiPlex Mix Ready To Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. HOT FIREPol® MultiPlex Mix Ready To Load is not recommended for use in applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications. Properties Concentration: 5x Hot-start: yes, initial activation in 12-15 min Ready to load: yes Fidelity: 1 x Taq Cloning Type: T/A cloning Multiplexing: tested with up to 18 targets (depending on amplicon length) Amplicon Size: up to 5 kb Mix Components HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x Multiplex Buffer with 10 mM MgCl2: 1x PCR solution – 2 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP Bovine serum albumin (BSA) to enhance PCR reaction Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment Yellow dye: migration equivalent to 35-45 bp DNA fragment Compound that increases sample density for direct loading |
