04-11-00115-10, FIREPol® Master Mix (7.5 mM MgCl2), 10 ml, 2500 rxn

04-11-00115-10, FIREPol® Master Mix (7.5 mM MgCl2), 10 ml, 2500 rxn

04-11-00115-5, FIREPol® Master Mix (7.5 mM MgCl2), 5 ml, 1250 rxn

916,63 RON

PCR Master Mix for routine applications

  • suitable for templates up to 5 kb
  • reaction set-up and shipment without dry ice
  • all-in-one master mix format reduces pipetting errors and saves time
  • amplicons are suitable for Sanger sequencing post-PCR
SKU
SBD_04-11-00115-5

Properties

Concentration: 5x

Hot-start: no

Ready to load: no

Fidelity: same as Taq

Cloning Type: T/A Cloning

Amplicon Size: up to 5 kb

Mix Components

FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase

5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20

12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2

1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

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Price 770,28 RON (preturile sunt fara TVA)
Description

Properties

Concentration: 5x

Hot-start: no

Ready to load: no

Fidelity: same as Taq

Cloning Type: T/A Cloning

Amplicon Size: up to 5 kb

Mix Components

FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase

5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20

12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2

1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP