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04-11-00125, FIREPol® Master Mix (12.5 mM MgCl2), 1 ml, 250 rxn
186,41 RON
PCR Master Mix for routine applications
- suitable for templates up to 5 kb
- reaction set-up and shipment without dry ice
- all-in-one master mix format reduces pipetting errors and saves time
- amplicons are suitable for Sanger sequencing post-PCR
SKU
SBD_04-11-00125
Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water.
We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.
Properties
Concentration: 5x
Hot-start: no
Ready to load: no
Fidelity: same as Taq
Cloning Type: T/A Cloning
Amplicon Size: up to 5 kb
Mix Components
FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase
5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
| Price | 154,06 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water. We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications. Properties Concentration: 5x Hot-start: no Ready to load: no Fidelity: same as Taq Cloning Type: T/A Cloning Amplicon Size: up to 5 kb Mix Components FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase 5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2 1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP |
