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01-03-KIT-00500, TERMIPol® DNA Polymerase, 500 U
1.178,10 RON
DNA Polymerase for MALDI-TOF and other applications with unconventional nucleotides
Thermostable DNA polymerase for highly efficient incorporation of unconventional nucleotides.
- efficient incorporation of ddNTPs, acyNTPs, or fluorescent nucleotides
- the assay success rate of 99% in MALDI-TOF
- robust and reliable
- reaction set-up and shipment without dry ice
SKU
SBD_01-03-0500
TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms.
The enzyme has 5’→ 3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs, acyNTPs, fluorescent dNTPs and labeled ddNTPs).
Applications
MALDI-TOF mass spectrometry
Primer extension
Mass-array
Properties
Concentration: 5 U/µl
Hot-start: no
Error rate per nucleotide per cycle is app. 8 x 10-5
Estimated half life at 95ºC is 1.5 hours.
Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC.
Reagents
TERMIPol® DNA Polymerase
10x Reaction buffer C 500 mM Tris-HCl pH 9.5 at 25ºC
100 mM MgCl2
| Price | 990,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5’→ 3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs, acyNTPs, fluorescent dNTPs and labeled ddNTPs).
Applications MALDI-TOF mass spectrometry Primer extension Mass-array
Properties Concentration: 5 U/µl Hot-start: no Error rate per nucleotide per cycle is app. 8 x 10-5 Estimated half life at 95ºC is 1.5 hours. Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers. Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase. Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC.
Reagents TERMIPol® DNA Polymerase 10x Reaction buffer C 500 mM Tris-HCl pH 9.5 at 25ºC 100 mM MgCl2 |
