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01-06-KIT-02000, HOT TERMIPol® DNA Polymerase, 2000 U
5.628,70 RON
Hot-start DNA Polymerase for MALDI-TOF and other applications with unconventional nucleotides
Thermostable hot-start DNA polymerase for highly efficient incorporation of unconventional nucleotides.
- efficient incorporation of ddNTPs, acyNTPs, or fluorescent nucleotides
- the assay success rate of 99% in MALDI-TOF
- robust and reliable
- reaction set-up and shipment without dry ice
SKU
SBD_01-06-KIT-02000
Description
HOT TERMIPol® DNA polymerase is a chemically modified TERMIPol® DNA polymerase. At ambient temperature it is inactive, having no polymerase activity.
HOT TERMIPol® DNA polymerase is fully activated by a 12-15 minute incubation at 95ºC. This prevents the extension of non-specifically annealed primers. The enzyme contains 5’→3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs).
Applications
MALDI-TOF mass spectrometry
Primer extension
Mass-array
Properties
Concentration: 5 U/µl
Hot-start: yes, activated by 12-15 minutes at 95ºC
Error rate per nucleotide per cycle is app. 8 x 10-5
Estimated half life at 95ºC is 1.5 hours.
Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC.
Reagents
HOT TERMIPol® DNA Polymerase
10x Reaction buffer C: 500 mM Tris-HCl pH 9.5 at 25ºC
100 mM MgCl2
| Price | 4.730,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Description HOT TERMIPol® DNA polymerase is a chemically modified TERMIPol® DNA polymerase. At ambient temperature it is inactive, having no polymerase activity. HOT TERMIPol® DNA polymerase is fully activated by a 12-15 minute incubation at 95ºC. This prevents the extension of non-specifically annealed primers. The enzyme contains 5’→3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs).
Applications MALDI-TOF mass spectrometry Primer extension Mass-array
Properties Concentration: 5 U/µl Hot-start: yes, activated by 12-15 minutes at 95ºC Error rate per nucleotide per cycle is app. 8 x 10-5 Estimated half life at 95ºC is 1.5 hours. Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers. Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase. Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC.
Reagents HOT TERMIPol® DNA Polymerase 10x Reaction buffer C: 500 mM Tris-HCl pH 9.5 at 25ºC 100 mM MgCl2 |
