Used for detecting double-strand DNA and single-stranded RNA bands as they separate during agarose gel electrophoresis.
Isolation of DNA fragments for subcloning without introducing mutations normally caused by EtBr.
Alternative to the ethidium bromide staining. As sensitive as EtBr or more sensitive than that.
Non-toxic, non-mutagenic and non-carcinogenic.
No hazard waste.
Stored at room temperature. This product has a stable shelf-life of 2 years.
RedSafeTM Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid stain, an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA.
This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of RedSafeTM bound to DNA is centered at 537 nm. RedSafeTM Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr.
The staining protocol for RedSafeTM Nucleic Acid Staining Solution (20,000x) is similar to that for EtBr.
Compared to EtBr, known as a strong mutagen, RedSafe Nucleic Acid Staining Solution (20,000x) causes much fewer mutations in the Ames test.
In addition, ResSafeTM Nucleic Acid Staining Solution (20,000x) has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test.
So it is wise to choose RedSafeTM Nucleic acid Stainig Solution (20,000x) instead of EtBr for detecting nucleic acid in agarose gels.
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