The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume.
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
|Processing Time||15 min|
|DNA Purity||Eluted DNA is ultrapure, endotoxin-free, and well suited for transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications. Typical Abs260/280 ≥ 1.8 and Abs260/230 ≥ 2.0|
|Plasmid DNA Size||Up to 25 kb|
|Plasmid DNA Yield||Up to 100 µg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)|
|Recovery Volume||≥ 25 µl of ZymoPURE™ Elution Buffer or DNase free water|
|Equipment Needed||Microcentrifuge and/or vacuum manifold (recommended).|
Plasmid DNA yield and concentration from the ZymoPure™ Miniprep Kit compared to other major suppliers. Plasmid DNA (pGL3®) was isolated from 5 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).