The ZymoPURE - Express™ Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without pelleting and resuspension steps. Just add the ZP Express™Lysis Buffer directly to your bacterial culture, neutralize, filter debris, and purify using the patented Zymo-Spin™ V-P microcentrifuge column.
The ZymoPURE - Express™ Plasmid Midiprep Kit is the fastest and easiest method for isolating plasmid DNA from E. coli. Innovative binding chemistry (patent pending) enables highly concentrated (up to 1.5 µg/µl) DNA to be eluted directly from microcentrifuge column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, and protein. The result is plasmid DNA ideal for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
As an added convenience, the ZymoPURE - Express™ Plasmid Midiprep Kit contains patented colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization and syringe filters are included for rapid lysate clearing.
No large centrifuge!
No cell pelleting!
No gravity filtration!
No ethanol precipitation!
From culture to endotoxin-free plasmid DNA in 18 minutes!
|Processing Time||18 min|
|Equipment||Microcentrifuge and vacuum/vacuum manifold|
|DNA Purity||Eluted DNA is ultrapure, endotoxin-free, and well suited for transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications. Typical Abs260/280 ≥ 1.8 and Abs260/230 ≥ 2.0|
|Plasmid DNA Size||Up to 25 kb|
|Plasmid DNA Yield||Up to 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)|
|Recovery Volume||≥ 200 µl of ZymoPURE™ Elution Buffer or DNase free water|
|Recommended Sample Volume||25 ml E. Coli Culture|