ZymoPURE™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. The ZymoPURE™ Plasmid Maxiprep kit features a spin column-based method for the purification of up to 1.2 mg of high-quality plasmid DNA in less than 18 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.
As an added convenience, the ZymoPURE™ Plasmid Maxiprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.
|Processing Time||18 min|
|Equipment||Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge.|
|DNA Purity||Eluted DNA is ultrapure and well suited for transfection, sequencing, cloning, plasmid mediated gene silencing, in vitro transcription, and other sensitive applications.|
|Plasmid DNA Size||Up to 25 kb|
|Plasmid DNA Yield||Up to 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)|
|Recovery Volume||≥ 200 µl of ZymoPURE™ Elution Buffer or DNase free water|
|Recommended Sample Volume||150 ml E. coli Culture|
Yield, concentration and transfection efficiency for plasmid DNA isolated using the ZymoPURE™ Maxiprep kit compared to two separate kits from Supplier Q. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). HeLa cells cultured in a 96-well plate were transfected with 100 ng of pGL3® Luciferase Reporter Vector using Lipofectamine® 2000 and luciferase expression was measured 48 hours later with the ONE-Glo™ Luciferase Assay System and Veritas™ Microplate Luminometer. Shown are means ± SEM of 8 transfections.
Plasmid DNA yield and concentration from the ZymoPure™ Maxiprep kit compared to other major suppliers. Plasmid DNA (pGEM®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).