The ZymoPURE-EndoZero™ Plasmid Gigaprep kit features a spin column-based method for the purification of up to 10 mg of high-quality endotoxin-free plasmid DNA in less than 50 minutes. The isolated plasmid DNA is ready for immediate use, and does not require subsequent precipitation steps.
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the kit incorporates our unique EndoZero™ spin-columns to reduce endotoxin levels down to 0.025 EU/µg of plasmid DNA without lengthy incubations, gravity flow anion-exchange columns, expensive chromatography columns, or time-consuming centrifugation steps. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, in vivo studies, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
As an added convenience, the ZymoPURE-EndoZero™ Plasmid Gigaprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
|Processing Time||50 min|
|Equipment||Vacuum/vacuum manifold and swinging bucket centrifuge.|
|DNA Purity||Eluted DNA is ultrapure, endotoxin-free, and well suited for transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, and other sensitive applications. Abs260/280 ≥ 1.8 and Abs260/230 ≥ 2.0. Endotoxin levels ≤ 0.025 EU/µg of plasmid DNA.|
|Plasmid DNA Size||Up to 25 kb|
|Plasmid DNA Yield||Up to 10 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)|
|Recovery Volume||≥ 2 ml of ZymoPURE™ Elution Buffer or DNase free water|