The Quick-RNA™ Whole Blood kit utilizes DNA/RNA Shield™, a unique preservation and lysis technology, to enable rapid isolation of total RNA from whole or partitioned blood or a cell pellet (after red blood cell lysis). The procedure uses Zymo-Spin™ column technology in which the sample is pre-filtered on the Zymo-Spin™ IIICG Column and then purified and concentrated on the Zymo-Spin™ IC Column. RNA is eluted into ≥ 6 µl of RNase-free water and is ready for any downstream application including RT-PCR, sequencing, etc.
|Sample Sources||Up to 1 ml mammalian whole-blood (fresh or stored in DNA/RNA Shield™ 2X concentrate), plasma, or serum. Also compatible with pelleted blood cells (PBMCs, WBCs, buffy coat, pelleted samples from PAXgene™ Blood RNA Tube, etc.) and nucleated blood.|
|RNA Purity||A260/A280 >1.8, A260/A230 >1.8. DNase I provided for complete removal of DNA.|
|RNA Recovery||RNA yields are species and sample/donor dependent and averages can range from 2-10 µg (1 ml human blood).|
|RNA Storage||RNA eluted with ≥6 µl RNase-free water can be stored at ≤-70 ºC.|
|Equipment Needed||Microcentrifuge and vortex.|
|RNA Size||RNAs ≥17 nucleotides.|
|Sample Preservation||DNA/RNA Shield™ effectively lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.|