Analiza Modificarilor Epienetice a Cromatinei

Epigenetic research addresses the alterations to the chromatin template that collectively establishes and propagates different patterns of transcription or gene expression and silencing from the same genome.

The major components of chromatin are DNA and histone proteins. Two copies of each histone core protein (H2A, H2B, H3 & H4) are assembled into an octamer that has 145- 147 base pairs of DNA wrapped around it to form a nucleosome core. The nucleosome controls the accessibility of DNA to the transcription machinery and chromatin remodelling factors (Luger et al. 1997).



    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR...

    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomic researches, namely to investigate protein-DNA interaction on a genome-wide scale. This techni...

    The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, Diagenode's Bioruptor® uses state-of-the-...

Made for Epigenetics Chromatin Analysis

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    iDeal ChIP-seq kit for Transcription Factors
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    IP-Star® Compact Automated System
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