Trypan Blue Stain is a stain used to distinguish viable from nonviable cells. Nonviable cells will absorb the dye and appear blue, while viable cells will exclude the dye. Cells should be in a single cell suspension in buffered saline before counting. Since trypan blue has a higher affinity for serum protein than for cellular proteins, suspending the cells in medium containing serum will generate a dark background. Commonly used to assess cell viability.
|Trypan Blue 0.4% solution in 0.85% NaCl, 100 ml||17-942E||EA||SDS|