X-VIVO™ Chemically Defined, Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL). These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes. The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and pasteurized human transferrin. All X-VIVO™ Media products are manufactured under current GMPs and are listed with the FDA in a product Master File. Permission to cross-reference the Master File may be obtained by contacting the Regulatory Affairs Department.
X-VIVO™ 10 Chemically Defined, Serum-free Hematopoietic Cell Media
The X-VIVO™ 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peri pheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2. X-VIVO™ 10 Media is available as a 1X liquid in two convenient formulations.
X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Media
X-VIVO™ 15 Media are similar in composition to X-VIVO™ 10 Media and have been optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. X-VIVO™ 15 Media support the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors. X-VIVO™ 15 Media can also be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells. In addition, X-VIVO™ 15 Media provide a serum-free environment for the expansion of HUT-78 and related human lymphocytic cell lines.
X-VIVO™ 20 Chemically Defined, Serum-free Hematopoietic Cell Medium
X-VIVO™ 20 Medium is optimized to support the generation of lymphokine activated killer (LAK) cells from monocytedepleted peripheral blood lymphocytes (PBL) at high density. Initial cell densities between 2.0 – 3.0 × 107 cells/ ml can be used to successfully generate LAK cells. X-VIVO™ 20 Medium may also be used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).
– Proliferation of peripheral blood lymphocytes
– Proliferation of tumor infiltrating lymphocytes
– Cryopreservation of human tissue
– Cultivation of human monocytes and macrophages
– Cultivation of stem cells
– Cultivation of dendritic cells