Adult melanocytes in MGM™-4 BulletKit™ seen under light microscopy
Most Clonetics™ Primary Cells are also available as proliferating cells in either flasks or plates, or as pellets in RNALater®.
Clonetics™ Adult and Neonatal Human Epidermal Melanocytes (NHEM) are the pigment producing cells located in the epidermal layers of all mammalian skin. The function of the melanocyte is to produce melanin, the pigment that gives skin its color. Melanin protects skin from UV damage. Skin is comprised of 2 main layers, the epidermis and the dermis. The epidermis is made of multiple keratinocyte layers. Melanocytes reside in the basal layer of the epidermis. The stellar or dendritic melanocyte morphology allows the melanocyte cell body to reside in the basal layer of the epidermis while the dendrites associate with the keratinocytes in the upper layers of the epidermis. This association allows the melanocyte to transport melanin from the cell body through the dendrites and into the keratinocytes resulting in the coloration of skin.
There are 2 main areas of melanocyte research - cosmetic and medical. Cosmetic studies center focus on the ability to change color of the skin. Medical research often centers on melanoma as well as various dermatological diseases or related disorders like albinism and vitiligo, hormonal treatments, thyroid conditions, Psoriasis, burns and scars.
Cryopreserved Clonetics™ Normal Human Epidermal Melanocytes are guaranteed through 8 population doublings, express Mel-5 and L-Dopa conversion to melanin. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Clonetics™ MGM™-4 Melanocyte Growth Medium-4 is recommended media for melanocyte optimal growth and survival in culture. This medium is offered in our traditional BulletKit™ format, providing flexibility to add or omit components as research needs dictate. ET-3 Growth Supplement, an additional growth factor required for adult melanocyte growth, is sold separately to supplement the MGM™-4 BulletKit™ for a complete formulation specifically designed for growing adult melanocytes. ET-3 offers enhanced growth and plating advantages in addition to limiting other cell type contamination in culture.
Note: Melanocytes are extremely sensitive to over trypsinization. Use Clonetics™ Trypsin/EDTA Solution (CC-5012) and dilute 1:1 with Versene®(17-711E) prior to use.
|Human Adult Melanocytes cryopreserved amp||CC-2586||EA||SDS|
|Neonatal Melanocytes (NHEM-Neo), MGM-4||CC-2504||EA||SDS|
|MGM-4 BulletKit (CC-3250 & CC-4435)||CC-3249||KT||SDS|
|MBM-4 Basal Medium 500 ml||CC-3250||EA||SDS|
|MGM-4 SingleQuot Kit Suppl. & Growth Factors||CC-4435||EA||SDS|
|Endothelin 3 (ET3) Lyophilized||CC-4510||EA||SDS|