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D5042, EZ-96 DNA Methylation-Gold MagPrep (4x96 rxns.)

D5042, EZ-96 DNA Methylation-Gold MagPrep (4x96 rxns.)

D5043, EZ-96 DNA Methylation-Gold MagPrep (8x96 rxns.)

8.538,25 RON

The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

SKU
ZR_D5043
Adaugati pentru comparare
HIGHLIGHTS
  • Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours.
  • A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
  • High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
DESCRIPTION

 

The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

For automation scripts and support, email automation@zymoresearch.com

 

TECHNICAL SPECIFICATIONS
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99%
Elution Volume ≥ 25 µl
Equipment Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.
Input 500 pg - 2 µg of DNA.
Processing Time 3 hours
Recovery > 70%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info
Mai multe informatii
Price 7.175,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS
  • Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours.
  • A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
  • High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.
DESCRIPTION

 

The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

For automation scripts and support, email automation@zymoresearch.com

 

TECHNICAL SPECIFICATIONS
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99%
Elution Volume ≥ 25 µl
Equipment Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.
Input 500 pg - 2 µg of DNA.
Processing Time 3 hours
Recovery > 70%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info
Manufacturer Zymo Research
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