The EZ DNA Methylation-Startup Kit provides the necessary tools for complete bisulfite conversion of DNA for methylation analysis. This kit includes reagents that allow for bisulfite conversion of purified DNA and DNA directly from blood, cells, and fresh or FFPE tissues without the prerequisite for upstream DNA purification. A fully methylated Universal Methylated Human DNA Standard is provided with a special primer set for PCR to assess conversion efficiency. Finally, a unique ZymoTaq DNA Polymerase is included for robust amplification of bisulfite-treated DNA.
The complete solution for bisulfite conversion. This all-in-one kit contains reagents for bisulfite conversion of DNA without the need for purification, methylated human DNA with control primers, and a robust hot-start PCR polymerase that is specifically formulated for bisulfite-converted DNA.
Designed for the first-time user requiring a consolidated product to control for bisulfite conversion.
Kit includes: one EZ DNA Methylation-Direct kit, one fully methylated Universal Methylated Human DNA Standard, and ZymoTaq DNA Polymerase.
DESCRIPTION
The EZ DNA Methylation-Startup Kit provides the necessary tools for complete bisulfite conversion of DNA for methylation analysis. This kit includes reagents that allow for bisulfite conversion of purified DNA and DNA directly from blood, cells, and fresh or FFPE tissues without the prerequisite for upstream DNA purification. A fully methylated Universal Methylated Human DNA Standard is provided with a special primer set for PCR to assess conversion efficiency. Finally, a unique ZymoTaq DNA Polymerase is included for robust amplification of bisulfite-treated DNA.
TECHNICAL SPECIFICATIONS
Applicable For
Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Applications
Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.
Conversion
> 99.5%
Elution Volume
≥ 10 µl
Equipment
Thermocycler with heated lid and microcentrifuge.
Input
Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells.
The complete solution for bisulfite conversion. This all-in-one kit contains reagents for bisulfite conversion of DNA without the need for purification, methylated human DNA with control primers, and a robust hot-start PCR polymerase that is specifically formulated for bisulfite-converted DNA.
Designed for the first-time user requiring a consolidated product to control for bisulfite conversion.
Kit includes: one EZ DNA Methylation-Direct kit, one fully methylated Universal Methylated Human DNA Standard, and ZymoTaq DNA Polymerase.
DESCRIPTION
The EZ DNA Methylation-Startup Kit provides the necessary tools for complete bisulfite conversion of DNA for methylation analysis. This kit includes reagents that allow for bisulfite conversion of purified DNA and DNA directly from blood, cells, and fresh or FFPE tissues without the prerequisite for upstream DNA purification. A fully methylated Universal Methylated Human DNA Standard is provided with a special primer set for PCR to assess conversion efficiency. Finally, a unique ZymoTaq DNA Polymerase is included for robust amplification of bisulfite-treated DNA.
TECHNICAL SPECIFICATIONS
Applicable For
Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
Applications
Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.
Conversion
> 99.5%
Elution Volume
≥ 10 µl
Equipment
Thermocycler with heated lid and microcentrifuge.
Input
Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells.