D5014-1,   Human HCT116 DKO Non-Methylated DNA (5 ug / 20 ul)

D5014-1, Human HCT116 DKO Non-Methylated DNA (5 ug / 20 ul)

D5015,   Bisulfite-Converted Universal Methylated Human DNA Standard

D5015, Bisulfite-Converted Universal Methylated Human DNA Standard

D5014-2, Human HCT116 DKO Methylated DNA (5 ug / 20 ul)

1.767,15 RON

The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation family of products. This DNA set can be included as a positive and negative control to assess the efficiency of bisulfite conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites.

SKU
ZR_D5014-2
HIGHLIGHTS

  • Purified, non-methylated and methylated human DNA for use as negative and positive controls in methylation detection applications. Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
  • Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP).
  • Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion
DESCRIPTION

The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation family of products. This DNA set can be included as a positive and negative control to assess the efficiency of bisulfite conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites.

TECHNICAL SPECIFICATIONS

Concentration 250 ng/µl
Format Purified genomic DNA
Storage ≤ -20°C
Supplemental Info
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Price 1.485,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS

  • Purified, non-methylated and methylated human DNA for use as negative and positive controls in methylation detection applications. Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
  • Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP).
  • Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion
DESCRIPTION

The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation family of products. This DNA set can be included as a positive and negative control to assess the efficiency of bisulfite conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites.

TECHNICAL SPECIFICATIONS

Concentration 250 ng/µl
Format Purified genomic DNA
Storage ≤ -20°C
Supplemental Info
Manufacturer Zymo Research