D5013-1,   Human WGA Non-Methylated DNA (5 ug / 20 ul)

D5013-1, Human WGA Non-Methylated DNA (5 ug / 20 ul)

D5014,   Human Methylated & Non-Methylated DNA Set (DNA w/ primers)

D5014, Human Methylated & Non-Methylated DNA Set (DNA w/ primers)

D5013-2, Human WGA Methylated DNA (5 ug / 20 ul)

1.463,70 RON

The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.

SKU
ZR_D5013-2
HIGHLIGHTS

  • Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications.
  • Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
  • Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP). Primers included with the full set are designed to amplify non-methylated, methylated, and mixed methylated copies of the Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene following bisulfite conversion.
DESCRIPTION

The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.

TECHNICAL SPECIFICATIONS

Concentration 250 ng/µl in buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
Sample Source Whole genome amplified DNA from HCT116 DKO cells. Human WGA Methylated DNA is enzymatically methylated by M.SssI methyltransferase.
Sample Storage ≤ -20°C
Supplemental Info
Mai multe informatii
Price 1.230,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS

  • Purified, non-methylated and methylated human DNA for use as negative and positive control in methylation detection applications.
  • Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
  • Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP). Primers included with the full set are designed to amplify non-methylated, methylated, and mixed methylated copies of the Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene following bisulfite conversion.
DESCRIPTION

The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, EZ DNA Methylation-Direct, and EZ DNA Methylation-Lightning kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA. The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.

TECHNICAL SPECIFICATIONS

Concentration 250 ng/µl in buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
Sample Source Whole genome amplified DNA from HCT116 DKO cells. Human WGA Methylated DNA is enzymatically methylated by M.SssI methyltransferase.
Sample Storage ≤ -20°C
Supplemental Info
Manufacturer Zymo Research