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D5008, EZ-96 DNA Methylation-Gold™ Kit (Deep-Well) (2 x 96 Rxns)
3.427,20 RON
The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion of unmethylated cytosines into uracil in less than 3 hours. Our optimized bisulfite chemistry combines heat denaturation and bisulfite conversion of input DNA to reduce incubation time and ensure conversion rates >99%. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
SKU
ZR_D5008
HIGHLIGHTS
- High-throughput (96-well) bisulfite conversion of DNA in less than 3 hours. A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
- Desulphonation and recovery of bisulfite-treated DNA with a 96-well plate.
- Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array. |
|---|---|
| Conversion | >99% |
| Elution Volume | ≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well |
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers |
| Input | 500 pg - 2 µg of DNA. |
| Processing Time | 3 hours |
| Recovery | >75% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info |
| Price | 2.880,00 RON (preturile sunt fara TVA) | ||||||||||||||||||
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| Description |
HIGHLIGHTS
DESCRIPTIONTECHNICAL SPECIFICATIONS
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| Manufacturer | Zymo Research |
