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D5003-1, CT Conversion Reagent (96 conversions) (1 Bottle)

D5003-1, CT Conversion Reagent (96 conversions) (1 Bottle)

D5003, EZ-96 DNA Methylation™ Kit (Shallow-Well) (2 x 96 Rxns)

3.332,00 RON
The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
SKU
ZR_D5003
Adaugati pentru comparare
HIGHLIGHTS
  • Streamlined, proven procedure for bisulfite conversion of DNA.
  • Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.
  • Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION

 

The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

Note: Catalog # D5004 is recommended for use with the Illumina Infinium MethylationEPIC BeadChip array.

 

TECHNICAL SPECIFICATIONS
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion >99%
Elution Volume ≥ 15 µl for Deep-well
≥ 30 µl for Shallow-well
Equipment Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input 500 pg - 2 µg of DNA
Processing Time 12-16 hours
Recovery >80%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info
Mai multe informatii
Price 2.800,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS
  • Streamlined, proven procedure for bisulfite conversion of DNA.
  • Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.
  • Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION

 

The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

Note: Catalog # D5004 is recommended for use with the Illumina Infinium MethylationEPIC BeadChip array.

 

TECHNICAL SPECIFICATIONS
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion >99%
Elution Volume ≥ 15 µl for Deep-well
≥ 30 µl for Shallow-well
Equipment Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input 500 pg - 2 µg of DNA
Processing Time 12-16 hours
Recovery >80%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info
Manufacturer Zymo Research
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