D4049,   ZR BAC DNA Miniprep Kit (100 Preps)

D4049, ZR BAC DNA Miniprep Kit (100 Preps)

D4048, ZR BAC DNA Miniprep Kit (25 Preps)

886,55 RON

The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.

SKU
ZR_D4048
HIGHLIGHTS

  • High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
  • Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization
  • Unique column design: Zero buffer retention and low elution volume (10 µl)
DESCRIPTION

 

The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.

 

TECHNICAL SPECIFICATIONS

Applicable For Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications.
Elution Volume ≥ 10 µl
Equipment Microcentrifuge
Processing Time 15 min
Purity Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0
Size Range Up to ~200 kb
Yield Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture.
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Price 745,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS

  • High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
  • Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization
  • Unique column design: Zero buffer retention and low elution volume (10 µl)
DESCRIPTION

 

The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.

 

TECHNICAL SPECIFICATIONS

Applicable For Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications.
Elution Volume ≥ 10 µl
Equipment Microcentrifuge
Processing Time 15 min
Purity Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0
Size Range Up to ~200 kb
Yield Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture.
Manufacturer Zymo Research