D4042,   Zyppy-96™ Plasmid Kit (4 x 96 Preps)

D4042, Zyppy-96™ Plasmid Kit (4 x 96 Preps)

D4043, Zyppy-96™ Plasmid Kit (8 x 96 Preps)

10.585,05 RON

The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.

SKU
ZR_D4043
HIGHLIGHTS

  • Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
  • High Quality: Ready for PCR, sequencing, cloning, and transfection.
  • Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
DESCRIPTION

The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.

TECHNICAL SPECIFICATIONS

Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume ≥ 30 µl
Equipment Centrifuge with micro plate carriers
Processing Time 45 min
Purity Typical Abs 260/280 ≥ 1.8.
Size Range Up to 25 kb
Yield Up to 5 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.
Supplemental Info
Mai multe informatii
Price 8.895,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS

  • Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
  • High Quality: Ready for PCR, sequencing, cloning, and transfection.
  • Streamlined Protocol: Culture, lyse, and neutralize in the same plate, saving time and reducing plastic waste.
DESCRIPTION

The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.

TECHNICAL SPECIFICATIONS

Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume ≥ 30 µl
Equipment Centrifuge with micro plate carriers
Processing Time 45 min
Purity Typical Abs 260/280 ≥ 1.8.
Size Range Up to 25 kb
Yield Up to 5 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.
Supplemental Info
Manufacturer Zymo Research