D2002, YeaStar™ Genomic DNA Kit (40 Preps) w/ Zymo-Spin™ III Columns (Uncapped)[Includes E1006 x 1: R-Zymolyase (1000 Units Lyophilized) w/ Storage Buffer (500 µl)]
1.130,50 RON
The YeaStar Genomic DNA Kit is designed for reliable and efficient isolation of genomic DNA from a broad spectrum of fungal species, including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The kit is based on highly efficient enzyme lysis and Zymo-Spin column technology. Each standard prep yields about 7 - 20 µg of DNA with a size distribution of 35 - 60 kb. The resulting genomic DNA can be used for direct analysis including Southern blotting, PCR, restriction endonuclease digestion, etc.
Simple: Fast spin-column procedure yields pure yeast genomic DNA without using glass beads or phenol.
Versatile: Efficient DNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
High-Quality: Isolated genomic DNA is ready for Southern blotting, PCR, restriction enzyme digestions, etc.
DESCRIPTION
The YeaStar Genomic DNA Kit is designed for reliable and efficient isolation of genomic DNA from a broad spectrum of fungal species, including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The kit is based on highly efficient enzyme lysis and Zymo-Spin column technology. Each standard prep yields about 7 - 20 µg of DNA with a size distribution of 35 - 60 kb. The resulting genomic DNA can be used for direct analysis including Southern blotting, PCR, restriction endonuclease digestion, etc.
TECHNICAL SPECIFICATIONS
Applicable For
Isolated DNA is well suited for downstream applications such as Southern blotting, PCR, restriction endonuclease digestion, Next-Generation Sequencing and other sensitive applications.
Elution Volume
≥ 60 µl TE or DNase-free Water
Equipment
Microcentrifuge, heat block/bath
Processing Time
1.5 hours
Processing Volume
≤ 1.5 ml of Culture (1-5 x 107 cells)
Purity
Typical A260/A280 & A260/A230 ≥ 1.8
Sample Source
Cell Culture (Colonies/Patches or Liquid Culture)
Size Range
Capable of recovering 35-60 kb sized fragments of genomic and mitochondrial DNA.
Simple: Fast spin-column procedure yields pure yeast genomic DNA without using glass beads or phenol.
Versatile: Efficient DNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
High-Quality: Isolated genomic DNA is ready for Southern blotting, PCR, restriction enzyme digestions, etc.
DESCRIPTION
The YeaStar Genomic DNA Kit is designed for reliable and efficient isolation of genomic DNA from a broad spectrum of fungal species, including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The kit is based on highly efficient enzyme lysis and Zymo-Spin column technology. Each standard prep yields about 7 - 20 µg of DNA with a size distribution of 35 - 60 kb. The resulting genomic DNA can be used for direct analysis including Southern blotting, PCR, restriction endonuclease digestion, etc.
TECHNICAL SPECIFICATIONS
Applicable For
Isolated DNA is well suited for downstream applications such as Southern blotting, PCR, restriction endonuclease digestion, Next-Generation Sequencing and other sensitive applications.
Elution Volume
≥ 60 µl TE or DNase-free Water
Equipment
Microcentrifuge, heat block/bath
Processing Time
1.5 hours
Processing Volume
≤ 1.5 ml of Culture (1-5 x 107 cells)
Purity
Typical A260/A280 & A260/A230 ≥ 1.8
Sample Source
Cell Culture (Colonies/Patches or Liquid Culture)
Size Range
Capable of recovering 35-60 kb sized fragments of genomic and mitochondrial DNA.