For in vitro use only
HOT TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5’→3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs).
HOT TERMIPol® DNA polymerase is a chemically modified TERMIPol® DNA polymerase. At ambient temperatures it is inactive, having no polymerase activity. HOT TERMIPol® DNA polymerase is fully activated by a 8-12 minute incubation at 95ºC. This prevents extension of non-specifically annealed primers.
- Primer extension
- MALDI-TOF mass spectrometry
- Mass-array
5 U/µl
- HOT TERMIPol® DNA Polymerase
- 10x Reaction Buffer C
500 mM Tris-HCl pH 9.5 at 25ºC
- 100 mM MgCl2
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT TERMIPol® DNA polymerase.
Price | 1.800,00 RON (preturile sunt fara TVA) |
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Description |
Applications:- Primer extension - MALDI-TOF mass spectrometry - Mass-array Concentration:5 U/µl
Reagents Provided:- HOT TERMIPol® DNA Polymerase - 10x Reaction Buffer C - 100 mM MgCl2
Storage and Dilution Buffer:50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Shipping and Storage Conditions:Routine storage: -20ºC |