Applications:

High Resolution Melt (HRM)

Mix Compatibility:

- Bio-Rad: CFX96™ & CFX384™

- Qiagen: Rotor-Gene^® 6000

- Eppendorf: Mastercycler^®: ep realplex2 & ep realplex4

- Illumina: The Eco™

- Roche: LightCycler^® 480

Mix Composition:

- HOT FIREPol^® DNA Polymerase

- 5x EvaGreen^® HRM buffer

- 12.5 mM MgCl₂ 1x PCR solution – 2.5 mM 

- EvaGreen^® dye

- BSA

EvaGreen^® Dye:

EvaGreen^® is a DNA-binding dye with many features that make it a superior for HRM. Apart from having similar spectra, EvaGreen^® has three important features that set it apart from SYBR^® Green I: EvaGreen^® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen^® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR^® Green or FAM!

Shipping and Storage conditions:

Routine storage: -20ºC 
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® EvaGreen^® HRM Mix.

EvaGreen^® is a registered trademark of BIOTIUM, INC.  
This product is provided under an agreement between BIOTIUM, INC. and SBD and the manufacture, use, sale or import of this product is subject to one or more of pending patent applications owned by BIOTIUM and ALLELOGIC. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis.  The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes.  Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research.  For information on purchasing a license to this product for purposes other than research, contact BIOTIUM, INC., Business Development, 3423 Investment Blvd, Suite 8, Hayward, CA 94545 Tel: 510-265-1027, Fax: 510-265-1352.”