Limba
Romanian
Romanian
English|
08-14-00008, 5x HOT FIREPol Probe qPCR Mix Plus (ROX), 8 ML, 2,000 x 20 µL Rxns
1.677,90 RON
5x HOT FIREPol® Probe qPCR Mix Plus (ROX)
For in vitro use only
Description:
HOT FIREPol®Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
SKU
08-14-00008
Applications:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Mix Compatibility:
- Appilied Biosystems: QuantStudioTM 12K Flex, ViiATM7, 7900HT, 7500, 7700, StepOneTM & StepOnePlusTM
- Stratagene: MX3000PTM , MX3005PTM
Mix Composition:
- HOT FIREPol® DNA Polymerase
- 5x Probe qPCR buffer
- 15 mM MgCl2 1x PCR solution – 3 mM
- dNTPs
- ROX dye
ROX Dye:
ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.
Shipping and Storage Conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (ROX).
Applied BioSystems and ROX are trademarks of Applied Biosystems LLC, ViiA and QuantStudio are trademark of Life Technologies Corporation; Stratagene, MX3000P and MX3005P are trademarks of Stratagene Corporation.
| Price | 1.410,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Applications:- Detection and quantification of DNA and cDNA targets - Profiling gene expression - Microbial detection - Viral load determination Mix Compatibility:- Appilied Biosystems: QuantStudioTM 12K Flex, ViiATM7, 7900HT, 7500, 7700, StepOneTM & StepOnePlusTM - Stratagene: MX3000PTM , MX3005PTM Mix Composition:- HOT FIREPol® DNA Polymerase - 5x Probe qPCR buffer - 15 mM MgCl2 1x PCR solution – 3 mM - dNTPs
- ROX dye
ROX Dye:ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR. Shipping and Storage Conditions:Routine storage: -20ºC Applied BioSystems and ROX are trademarks of Applied Biosystems LLC, ViiA and QuantStudio are trademark of Life Technologies Corporation; Stratagene, MX3000P and MX3005P are trademarks of Stratagene Corporation. |
