04-12-00S15,    FIREPol 5x Master Mix 7,5 mM ready to load, 0,1 ML, 25 x 20 µL Rxns

04-12-00S15, FIREPol 5x Master Mix 7,5 mM ready to load, 0,1 ML, 25 x 20 µL Rxns

04-12-00115,    FIREPol 5x Master Mix 7,5 mM ready to load, 1 ML,  250 x 20 µL Rxns

04-12-00115, FIREPol 5x Master Mix 7,5 mM ready to load, 1 ML, 250 x 20 µL Rxns

04-12-00S25, FIREPol 5x Master Mix 12,5 mM ready to load, 0,1 ML, 25 x 20 µL Rxns

59,50 RON

5x FIREPol® Master Mix Ready to Load

with 12.5 mM MgCl2

For in vitro use only

 

Description: 

5x FIREPol® Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). 

We recommend using 5x FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5x FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.

 

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SKU
04-12-00S25

Applications:

- Suited for a wide range of PCR assays 

- Primer extension

 

Reagent Composition: 

- FIREPol® DNA polymerase 

- 5x Reaction Buffer B  0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20 

- 12.5 mM MgCl1x PCR solution –  2.5 mM MgCl

- 2 mM dNTPs of each 1x PCR solution – 200 µM dATP, 200 µM dCTP,   200 µM dGTP and 200 µM dTTP  

- Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment 

- Yellow dye  Migration rate in excess of primers in 1% agarose gel: <35-45 bp 

- Compound that increases sample density for direct loading 

Advantages of Ready-to-Load Mix:

- decreased set-up time 

- reduced risk of contamination 

- less pipetting mistakes 

- consistent reaction to reaction performance 

- contains all reagents required for PCR 

Like all our products, the 5x FIREPol® Master Mix Ready to Load is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.

 

Shipping and Storage Conditions:

Routine storage: -20ºC 
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of FIREPol® 5x Master Mix Ready to Load.

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Description

Applications:

- Suited for a wide range of PCR assays 

- Primer extension

 

Reagent Composition: 

- FIREPol® DNA polymerase 

- 5x Reaction Buffer B  0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20 

- 12.5 mM MgCl1x PCR solution –  2.5 mM MgCl

- 2 mM dNTPs of each 1x PCR solution – 200 µM dATP, 200 µM dCTP,   200 µM dGTP and 200 µM dTTP  

- Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment 

- Yellow dye  Migration rate in excess of primers in 1% agarose gel: <35-45 bp 

- Compound that increases sample density for direct loading 

Advantages of Ready-to-Load Mix:

- decreased set-up time 

- reduced risk of contamination 

- less pipetting mistakes 

- consistent reaction to reaction performance 

- contains all reagents required for PCR 

Like all our products, the 5x FIREPol® Master Mix Ready to Load is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.

 

Shipping and Storage Conditions:

Routine storage: -20ºC 
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of FIREPol® 5x Master Mix Ready to Load.