For in vitro use only
FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol®has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.
- Suited for a wide range of PCR assays
- Primer extension
- TA cloning
5 U/µl
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
- FIREPol® DNA Polymerase
- 10x Reaction buffer B (Mg2+ free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20
- 10x Reaction buffer BD (Mg2+ and detergent free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4
- 25 mM MgCl2
- 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol®DNA Polymerase.
Price | 195,00 RON (preturile sunt fara TVA) |
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Description |
Applications:- Suited for a wide range of PCR assays - Primer extension - TA cloning Concentration:5 U/µl Storage and Dilution Buffer:50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents Provided:- FIREPol® DNA Polymerase - 10x Reaction buffer B (Mg2+ free) 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20 - 10x Reaction buffer BD (Mg2+ and detergent free) 0.8 M Tris-HCl, 0.2 M (NH4)2SO4 - 25 mM MgCl2 - 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution). Shipping and Storage Conditions:
Routine storage: -20 ºC |