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P2002, His-Spin Protein Miniprep™ Kit (50 Preps)
2.374,05 RON
The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 µl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.
SKU
ZR_P2002
HIGHLIGHTS
- Fast: Purify His-tagged proteins from cell lysates in less than 5 minutes.
- Simple: Prepare pure protein for small-scale studies using a spin-column.
- Convenient: No special instrumentation needed other than a benchtop microcentrifuge.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Affinity Matrix | Nickel charged agarose |
|---|---|
| Binding Capacity | 1 mg |
| Elution Method | Imidazole gradient |
| Elution Volume | 100 - 200 µl (150 µl is optimal) |
| Principle of Technology | Based on a nickel-charged His-Affinity Gel (IMAC). |
| Processing Time | 5 minutes |
| Product Storage | Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature. |
| Protein Purity | Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications. |
| Required Equipment | Microcentrifuge |
| Sample Type | Cell lysates or other complex protein mixtures containing His-tagged proteins. |
| Price | 1.995,00 RON (preturile sunt fara TVA) | ||||||||||||||||||||
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| Description |
HIGHLIGHTS
DESCRIPTIONTECHNICAL SPECIFICATIONS
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| Manufacturer | Zymo Research |
