Atlantis dsDNase is a double-stranded DNA-specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes.
Sold separately or as part of EZ Nucleosomal DNA Prep Kit (D5220).
DESCRIPTION
Atlantis dsDNase is a double-stranded DNA-specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes.
TECHNICAL SPECIFICATIONS
Concentration
0.1 U/µl
Inactivation
5X MN Stop Buffer or EDTA
Standard Reaction Time
20 minutes
Storage
Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤-70°C.
Unit Definition
One unit (U) is defined as the amount of enzyme needed to produce an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2 (Kunitz, 1950).
Sold separately or as part of EZ Nucleosomal DNA Prep Kit (D5220).
DESCRIPTION
Atlantis dsDNase is a double-stranded DNA-specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes.
TECHNICAL SPECIFICATIONS
Concentration
0.1 U/µl
Inactivation
5X MN Stop Buffer or EDTA
Standard Reaction Time
20 minutes
Storage
Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤-70°C.
Unit Definition
One unit (U) is defined as the amount of enzyme needed to produce an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2 (Kunitz, 1950).