E2026,   5-hmC Glucosyltransferase (100 U)

E2026, 5-hmC Glucosyltransferase (100 U)

M2001,   ZymoMag Protein A   (200 ul)

M2001, ZymoMag Protein A (200 ul)

E2027, 5-hmC Glucosyltransferase (200 U)

1.535,10 RON

The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a
glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection.

SKU
ZR_E2027
HIGHLIGHTS

  • Highly processive enzyme for specific modification of 5-hydroxymethylcytosine (5-hmC) with a glucose moiety.
  • Ideal for locus specific and global quantification of hydroxymethylated DNA.
DESCRIPTION

 

The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a
glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection.

 

TECHNICAL SPECIFICATIONS

Concentration 2 U/µl
Standard Reaction Time 2 hours
Storage 5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.
Unit Definition One unit (U) is defined as the amount of enzyme needed to protect 1 µg of 5-hmC DNA Standard [D5405-3] from Csp6I restriction enzyme digestion via glucosylation in a reaction incubated at 30°C for 1 hour.
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Price 1.290,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS

  • Highly processive enzyme for specific modification of 5-hydroxymethylcytosine (5-hmC) with a glucose moiety.
  • Ideal for locus specific and global quantification of hydroxymethylated DNA.
DESCRIPTION

 

The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a
glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection.

 

TECHNICAL SPECIFICATIONS

Concentration 2 U/µl
Standard Reaction Time 2 hours
Storage 5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.
Unit Definition One unit (U) is defined as the amount of enzyme needed to protect 1 µg of 5-hmC DNA Standard [D5405-3] from Csp6I restriction enzyme digestion via glucosylation in a reaction incubated at 30°C for 1 hour.
Manufacturer Zymo Research