E2020,   DNA Degradase Plus™ (250 U)

E2020, DNA Degradase Plus™ (250 U)

E2030,   Atlantis dsDNase 12.5 U (0.1 U/ul)

E2030, Atlantis dsDNase 12.5 U (0.1 U/ul)

E2021, DNA Degradase Plus™ (1000 U)

3.224,90 RON

DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.

SKU
ZR_E2021
HIGHLIGHTS

  • Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS
  • Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol
DESCRIPTION

 

DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.

 

TECHNICAL SPECIFICATIONS

Assay Condition DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour
Concentration 5 U/µl
Enzyme Inactivation Heat inactivate enzyme at 70C for 20 minutes
Storage Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.
Unit Definition 5 U of enzyme will digest 1 µg of genomic DNA in 25 µl reaction volume at 37C for ≥ 1 hr
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Price 2.710,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS

  • Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS
  • Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol
DESCRIPTION

 

DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.

 

TECHNICAL SPECIFICATIONS

Assay Condition DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour
Concentration 5 U/µl
Enzyme Inactivation Heat inactivate enzyme at 70C for 20 minutes
Storage Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.
Unit Definition 5 U of enzyme will digest 1 µg of genomic DNA in 25 µl reaction volume at 37C for ≥ 1 hr
Manufacturer Zymo Research