E2019-50,   dsDNA Shearase™ Plus 50 U (1 U/ul) + DCC-5 (D4013)

E2019-50, dsDNA Shearase™ Plus 50 U (1 U/ul) + DCC-5 (D4013)

E2020,   DNA Degradase Plus™ (250 U)

E2020, DNA Degradase Plus™ (250 U)

E2019-200, dsDNA Shearase™ Plus 200 U (1 U/ul)+ DCC-5 (D4014)

5.051,55 RON
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphates and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs thus minimizing sample loss.
SKU
ZR_E2019-200
HIGHLIGHTS

  • The simplest method for generating random-ended dsDNA fragments. Fragment size can be controlled by adjusting the enzyme concentration.
  • Included DNA Clean & Concentrator-5 (DCC-5) kit allows for easy clean-up of fragmented DNA.
  • Fragmented DNA is ideal for downstream applications such as library construction, Next-Gen sequencing, MeDIP, MeDIP-Seq, etc.
DESCRIPTION

 

dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphates and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs thus minimizing sample loss.

 

TECHNICAL SPECIFICATIONS

Concentration 1 U/µl
Enzyme Inactivation Heat in activate enzyme at 65C for 5 min.
Storage Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.
Unit Definition One unit (1 U) is defined at the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100-500 bp in 20 minutes at 42C in total reaction volume in 10 µl.
Mai multe informatii
Price 4.245,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS

  • The simplest method for generating random-ended dsDNA fragments. Fragment size can be controlled by adjusting the enzyme concentration.
  • Included DNA Clean & Concentrator-5 (DCC-5) kit allows for easy clean-up of fragmented DNA.
  • Fragmented DNA is ideal for downstream applications such as library construction, Next-Gen sequencing, MeDIP, MeDIP-Seq, etc.
DESCRIPTION

 

dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphates and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs thus minimizing sample loss.

 

TECHNICAL SPECIFICATIONS

Concentration 1 U/µl
Enzyme Inactivation Heat in activate enzyme at 65C for 5 min.
Storage Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.
Unit Definition One unit (1 U) is defined at the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100-500 bp in 20 minutes at 42C in total reaction volume in 10 µl.
Manufacturer Zymo Research