SePOP (Selective Precipitation Optimized Process) desalting is based on a differential precipitation process. It has been specifically developed by Eurogentec to increase the purity level of the deprotected and desalted Oligonucleotides from 10 to 59 bases.


SePOP (Selective Precipitation optimised Process) has been specifically developed to increase the purity level of the deprotected and desalted oligonucleotides. 

This technique is based on a differential precipitation process and allows purification of oligonucleotides from 10 to 59 bases. 

During SePOP desalting, the largest part of contaminants (truncated material < 10 bases) will be eliminated and the oligonucleotides will be segregated with respect to their biophysical properties. To achieve the optimum result, three parameters are strictly managed: temperature, time of precipitation and the nature of the salt used in the precipitation. The purity level up to 70 % obtained is therefore adequate for most common applications, such as routine PCR and hybridisation. 

 

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SePOP desalting

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