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2 O-Me RNA Bases
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Oligo-2’ O-Methyl (O-Me) nucleotides are resistant (but not totally resistant) to a variety of ribo- and deoxyribonucleases. As well as being stable to normal handling and nuclease resistant, oligo-2’ O-Me-nucleotides form more stable hybrids with complementary RNA strands than equivalent DNA and RNA sequences.
Oligo-2’ O-Methyl (O-Me) nucleotides are resistant (but not totally resistant) to a variety of ribo- and deoxyribonucleases. As well as being stable to normal handling and nuclease resistant, oligo-2’ O-Me-nucleotides form more stable hybrids with complementary RNA strands than equivalent DNA and RNA sequences. This combination of useful properties promises to make 2’ O-Me-RNA sequences powerful research tools. Conveniently, the synthesis and handling of 2’ O-Me RNA is as simple to perform as DNA synthesis, and avoids most of the problems associated with RNA synthesis.
The combination of properties offered by oligo-2’ O-Me-nucleotides (nuclease resistance, stable hybrid formation, ease of synthesis) make them especially suitable for use as antisense probes. In this mode, RNase H resistant oligo-2’ O-Me-nucleotides, labelled or unlabelled, can be used to block specific RNA functions and, when attached to a suitable tag, usually biotin, can be used in affinity purification of RNA complexes.
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