The genomic DNA is isolated by cell lysis followed by the addition of Proteinase K and RNase A and purification on columns. The conditions have been chosen to allow for PCR or other enzymatic reactions in the downstream process. The fragment length of the purified DNA is up to 50kb.
Studies on gene expression.
The quality and quantity of the resulting DNA are determined by measuring the absorption at 260nm /280nm using the Nanodrop 1000. All data are between 1.8 und 2.0. As RNAse is added during the isolation, a contamination with RNA can be excluded.