The Femto™ Human DNA Quantification Kit can detect and quantify human DNA with high specificity and sensitivity. Human DNA can be reliably quantified in a background of non-human DNA such as bacterial, fungal, animal, plant DNA, etc. This is essential for downstream applications that require accurate DNA input amounts such as STR analysis. With the Femto™Human DNA Quantification Kit, dependably quantify as little as 20 fg from 1 µl of purified biological liquids, anthropological samples, or forensic DNA samples.
|Sample Sources||Detect and quantify high quality human DNA from any purified mixed DNA sample.|
|Compatibility||Product is designed to be compatible with any real-time and quantitative PCR instrument.|
|Bacterial DNA Detection and Quantification||Detection range of 20 fg-20 ng from as little as 1 µl of sample. The kit can be used to detect less than 1 copy of human gDNA.|
Reliable standards for the quantification of human DNA: Human DNA Standards performed in duplicates 10-fold serial dilutions ranging from 20 ng to 20 fg.
Specificity: Amplification plots demonstrate the specificity of the Femto™ Human DNA Quantification Kit. Bovine gDNA, porcine gDNA, and murine gDNA exhibited delayed amplification similar to the No Template Control (black) (≥35 cycles). 0.2 ng of human brain gDNA (orange), human gDNA extracted from urine (gold), bovine gDNA (red), porcine gDNA (light blue), and murine gDNA (purple) were used.