The ZymoBIOMICS™-96 MagBead DNA Kit is designed for purifying DNA from a wide array of sample inputs (e.g. feces, soil, water, and biofilms) that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. gram negative/positive bacteria, fungi, protozoans, and algae), making it ideal for microbiomic studies. Unbiased mechanical lysis of tough microbe s is achieved by bead beating with Zymo Research’s proprietary, ultra-high density BashingBeads™ and validated using the ZymoBIOMICS™ Microbial Community Standard as shown in Figure 3. Coupling this with Zymo Research’s Purification and PCR Inhibitor Removal Technologies result in superior yields of ultra-pure DNA that is free of PCR inhibitors (e.g. polyphenols, humic and fulvic acids), making it ideal for all downstream applications including PCR, arrays, 16s rRNA Gene Sequencing, and Shotgun Sequencing.
|DNA Recovery||Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum) ZymoBIOMICS™ DNase/RNase Free Water.|
|Equipment||Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block|
|Sample Sources||Bacterial, fungal, protozoan, algal, viral, mitochondrial, and host DNA is efficiently isolated from ≤ 100 mg of mammalian feces, ≤ 200 mg soil, and 5 – 20 mg (wet weight) of fungal/bacterial cells, biofilms, and water|
|DNA Purity||High quality, inhibitor-free DNA is eluted with ZymoBIOMICS™ DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation Sequencing (NGS)|
|Bead Beating System||The ZymoBIOMICS™ innovative lysis system enables complete homogenization/disruption of the microbial cell walls and accurate microbial DNA analysis, free of bias. To ensure unbiased lysis, calibration of each bead-beating device is recommended by using the ZymoBIOMICS™ Microbial Community Standard|
|DNA Integrity||On average, post bead beating, genomic DNA is between 15-20 kb depending on the initial quality of the sample making it amenable to Next-Generation sequencing platforms requiring high molecular weight DNA. For optimal DNA integrity, collect samples in DNA/RNA Shield™|
|Bioburden||A single preparation is guaranteed to contain less than 3 bacterial genomic copies per 1 µl of eluate, as determined by quantitative amplification of the 16S rRNA gene when eluted using 100 µl water|