ZymoBIOMICS™ PCR Premix

ZymoBIOMICS™ PCR Premix

 Add water, DNA and primers and go!
 Robust amplification for the detection of low copy DNA
 Ideal for highly sensitive applications
 Certified low-bioburden
 
ProductSizeCatalog #
ZymoBIOMICS™ PCR Premix 50 Rxns E2056
ZymoBIOMICS™ PCR Premix 200 Rxns E2057


About ZymoBIOMICS™ PCR Premix

 

The ZymoBIOMICS™ PCR PreMix is supplied as a 2X concentrated “master mix" and contains all the reagentsneeded to perform PCR and other molecular downstreamanalysis with the addition of probes or fluorescent dyes. It features a hot-start DNA polymerase and is validated lowbioburdenin regards to bacterial contamination. Simple and easy to use, just add water, primers, and template DNA to the ZymoBIOMICS™ PCR PreMix and then heat at 95 ˚C for 10 minutes to initiate polymerization.

ZymoBIOMICS™ DNA polymerase is a heat-activated, “hotstart” polymerase that has 3’-terminal transferase activity. The addition of “A” overhangs to amplified DNA makes it ideal foruse in TA-cloning.


Figure 1. A tenfold serial dilution of Lactobacillus fermentum genomic DNA was quantified via real-time PCR, after the addition of 2.5 µM SYTO® 9 toa 50 µl reaction volume. Amplification of the 16S rRNA gene can be quantified down to 2 femtograms of bacterial genomic DNA in a 45 cycle qPCR.

Figure 2. Quantification of no template controls (NTCs) via real-time PCR was determined by amplification of the 16S rRNA gene, after the addition of 2.5 µM SYTO® 9 to a 50 µl reaction volume. Real-time PCR was performed for 45 cycles to determine the amount of bacterial contamination. NTCs include (A) Millipore filtered water and (B) DEPC treated Millipore filtered water.

Figure 3. Amplification of the 16S rRNA gene determined by quantitative PCR.

 

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