ZymoBIOMICS™ DNA/RNA Mini Kit

ZymoBIOMICS™ DNA/RNA Mini Kit

 

 Rapid, robust, and simple purification of high quality, inhibitor-free DNA and total RNA (including small/micro RNAs) from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.
 ZymoBIOMICS™ innovative lysis system enables efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungus, protozoans, algae, viruses, etc.
 High quality DNA and DNA-free RNA is ready for use in any downstream application. DNase I included.
 
ProductSizeCatalog #
ZymoBIOMICS™ DNA/RNA Mini Kit 50 Preps R2002
 

About ZymoBIOMICS™ DNA/RNA Mini Kit

 

The ZymoBIOMICS DNA/RNA Mini Kit is designed for purifying DNA and RNA from a wide array of sample inputs (e.g.feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. gram negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin® column technology that results in high-quality DNA and total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids) and is ready for RT-PCR, arrays, sequencing, etc.

 

Equipment Microcentrifuge, vortex, cell disrupter (recommended).
Sample Sources Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells1, biofilms, and water.
Storage DNA and RNA eluted with DNase/RNase-Free Water can be stored at ≤70ºC. The addition of RNase inhibitors in highly recommended for prolonged storage.
Purity High quality DNA and RNA (A260/A280 >1.8, A260/A230 >1.8) are recovered.
Size Limit Capable of recovering DNA and total RNA ≥17 nucleotides.
Recovery The DNA/RNA binding capacity of the Zymo-Spin™ IIICG Column is ~100 µg.
Sample Preservation DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.
Bead Beating System ZymoBIOMICS™ innovative lysis system ensures complete homogenization of the microbial cell walls and accurate microbial analysis, free of bias.

 

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